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rat anti caspase 11  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rat anti caspase 11
    Rat Anti Caspase 11, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+11/caspase-11+Antibody/pm41802485-102-46-50
    Average 93 stars, based on 61 article reviews
    rat anti caspase 11 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Hepatocyte‐Specific GSDMD Deficiency Aggravates Sepsis by Disrupting Non‐Canonical Secretion of Anti‐Inflammatory Factors
    Article Snippet: After the liver tissue was homogenized with RIPA lysis buffer (Beyotime Biotechnology, Shanghai, China), the supernatant was centrifuged at 13 000 rpm for 10 min, and Western blot was performed after BCA protein quantification. .. After preparing 10% gel, the gel was run at a constant voltage of 100V, followed by 400 mA transfer for 45 min. After being washed with TBST (Solarbio Life Sciences, Beijing, China) three times for 10 min each time, the membranes were blocked with 5% skimmed milk powder (Sangon Biotech, Shanghai, China) for 1 h. The membranes were incubated with GSDMD (abcam, #ab209845, Cambridge, UK), caspase‐1 (Santa Cruz Biotechnology, #sc‐56036, TX, USA), caspase‐11 (Santa Cruz Biotechnology, #sc‐374615, TX, USA), VEGF‐B (R&D Systems, #MAB751, MN, USA), Gremlin‐1 (CST, #4383, MA, USA) and other primary antibodies at 4 °C overnight. ..

    Article Title: Involvement of PRDX6 in the protective role of MANF in acute lung injury in rats.
    Article Snippet: .. Then, the PVDF membrane was incubated with primary antibodies, including PRDX6 (LFMA0018, Invitrogen), Caspase-11 (sc-374615, Santa Cruz Biotechnology), Gasdermin D N-terminal (GSDMD-N) (A20197, ABclonal Technology), and GAPDH (T0004, Affinity) overnight at 4 °C. ..

    Article Title: Restoration of the Mucosal IgA Response by Improving CD4 + T Pyroptosis Fails to Attenuate Gut Bacterial Translocation and Organ Damage After LPS Attack.
    Article Snippet: Background Currently, the mechanisms of impaired gut mucosal immunity in sepsis remain unclear.. Gut immunoglobulin A (IgA) is an important defense mechanism against invasive pathogens, and CD4+ T cells regulate the IgA response.. Aim We aimed to verify the hypothesis indicating that CD4+ T pyroptosis induced by lipopolysaccharide (LPS) leads to an impaired gut IgA response and subsequent bacterial translocation and organ damage.

    Membrane:

    Article Title: Involvement of PRDX6 in the protective role of MANF in acute lung injury in rats.
    Article Snippet: .. Then, the PVDF membrane was incubated with primary antibodies, including PRDX6 (LFMA0018, Invitrogen), Caspase-11 (sc-374615, Santa Cruz Biotechnology), Gasdermin D N-terminal (GSDMD-N) (A20197, ABclonal Technology), and GAPDH (T0004, Affinity) overnight at 4 °C. ..

    Article Title: Cardamonin Commands the Colonic Metabolite Columbianetin Acetate to Compete Against LPS in Binding HMGB1 in the Kidney, Preventing Lipopolysaccharide‐Induced Renal Tubular Pyroptosis
    Article Snippet: .. Antibodies used in this study include mouse β-tubulin (66240- 1-Ig, Proteintech, Wuhan, Hubei, China), apoptosis-associated speck-like protein containing a CARD (ASC, #67824, Cell Signaling Technology, Shanghai, China), Caspase-1 (AG-20B0042B, AdipoGen Life Sciences, Beijing, China), Caspase-11 (sc-374615, Santa Cruz Biotechnology, Shanghai, China), Cathepsin D (sc-377299, Santa Cruz Biotechnology), glyceraldehyde3-phosphate dehydrogenase (GAPDH, 60004-1-Ig, Proteintech), GSDMD (A20728, ABclonal, Wuhan, Hubei, China), HMGB1 (sc-56698, Santa Cruz Biotechnology), IL-1β (#12242, Cell Signaling Technology), interleukin 6 (IL-6, 21865-1-AP, Proteintech), lysosomal-associated membrane protein 1 (LAMP1, #14-1071-81, eBioscience, San Diego, CA, USA), Na+/K+ ATPase (A11683, ABclonal), NLR family pyrin domain containing 3 (NLRP3, #15101, Cell Signaling Technology), rabbit-anti-F4/80 (#70076, Cell Signaling Technology) and Alexa Fluor 488 conjugated goat anti-rabbit IgG (#111-545-144, Jackson ImmunoResearch, Shanghai, China). .. Recombinant HMGB1 protein (RP01545) was purchased from ABclonal.

    Western Blot:

    Article Title: Natterin bridges IFN-φ1 and non-canonical inflammasome pathways via CRFB1 /Gbp4 to license Caspy2-mediated antibacterial immunity
    Article Snippet: The bands were identified by Ponceau solution staining (Sigma, #P3504), and the membrane, previously washed with TBST solution, remained overnight in blocking solution (TBST-5% powdered milk). .. The specific proteins were detected in the iBindTM Flex Western System using iBind Flex Cards (Invitrogen, #SLF2010) and the solution made with the iBind solution Kit (Invitrogen, #SLF1020) and the primary antibodies: rabbit polyclonal IgG anti-mouse caspase-1 (p-10, M-20) (#sc-514, Santa Cruz Biotechnology, Dallas, United States, 1/200, detects the immature form with 50 kDa and the mature form around 28 kDa), rabbit polyclonal IgG raised against amino acids 301–350 of caspase-11 of mouse origin (p-10 (M-50; #sc-28231; Santa Cruz Biotechnology, 1/200, detects the immature form with 50 kDa and the mature form around 28 kDa) followed by rabbit HRP-labeled anti-rabbit IgG TrueBlot second antibody (Rockland, #18-8816–33 at 1/1,000) for 3h. .. Natterin was detected with rabbit anti-serum against natterin purified from Thalassophryne nattereri venom (dimeric form around 62 kDa; dilution: 1/50), followed by the second antibody anti-rabbit IgG HRP TrueBlot (#18-8816-33, Rockland; dilution 1/1,000).



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    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, <t>and</t> <t>Caspase-11</t> protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.
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    Image Search Results


    Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, and Caspase-11 protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.

    Journal: Balkan Medical Journal

    Article Title: Fucoxanthin Attenuates Bisphenol A-Induced Testicular Injury via NF-κB-Mediated Pyroptosis Inhibition

    doi: 10.4274/balkanmedj.galenos.2026.2025-11-268

    Figure Lengend Snippet: Effects of Fx on the pyroptosis signaling pathway in vivo . (a) RT-qPCR analysis of mRNA levels for Nlrp3 , Casp1 , and Gsdmd ; (b) representative immunofluorescence staining images in testicular sections for LPS (red) and Hoechst 33342-stained nuclei (blue) (400 ×), immunohistochemistry images showcasing NLRP3, Caspase-1, and Caspase-11 protein expression (400 ×) and their quantitative diagrams; (c) the expression of Caspase-1 p20/Caspase-1, Caspase-11 p20/Caspase-11, N-GSDMD and N-GSDMD/GSDMD protein levels in mice testis by immunoblotting assay and their quantitative diagrams. Data presentation and statistical analysis are as described for Figure 1 (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001. BPA, bisphenol A; Fx, fucoxanthin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; LPS, lipopolysaccharide; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3; GSDMD, gasdermin D.

    Article Snippet: Caspase-11 (NB120-10454) , Novus Biologicals (Littleton, USA).

    Techniques: In Vivo, Quantitative RT-PCR, Immunofluorescence, Staining, Immunohistochemistry, Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Binding Assay